Mechanisms of Retinal Ganglion Cell Death in Glaucoma

Principal Investigator

Detailed Non-Technical Summary


In a project funded previously by NGR, Dr. Agarwal created a retinal ganglion cell (RGC) line to be used in further research. He is now utilizing the cell line in an in-vitro experiment in which the cells are deprived of a growth factor to see how this affects apoptosis (cell death) in RGCs. His hypothesis is that growth factor deprivation will cause the cells to further decrease the production of autocrine (self-produced) growth factors. He will then attempt to determine if the in vitro model of cell death involves activation of the caspases that are thought to be central to RGC apoptosis in vivo. If the new cell line duplicates the known effects of growth factor deprivation already observed in animals, this cell line will be highly useful for further experimentation on the process of cell death in glaucoma.

Publications

Krishnamoorthy, R.R., Agarwal, P., Prasanna, G., Vopat, K., Lambert, W., Sheedlo, H.J., Pang, I.H., Shade, D., Wordlinger, R.J., Yorio, T., Clark, A., and Agarwal, N. (2001) Characterization of a transformed rat retinal ganglion cell line. Mol. Brian Res. 86:1-12.  
 

First published on: June 11, 2008

Last modified on: July 06, 2022