A Novel Antioxidant Therapy for Retinal Degeneration
The goal of this project is to develop a novel drug to prevent geographic atrophy, the advanced form of dry AMD. Based on considerable biochemical and clinical data, we hypothesize that with age, normal metabolic activity produces reactive forms of oxygen that damage the retina. We plan to test a newly discovered compound to prevent this damage in a mouse model of dry AMD in which the build up of reactive oxygen molecules is accelerated. This drug was selected in a high throughput screen of over 1.3 million compounds for its ability to stimulate the production of protective enzymes.
In our current experiments, we will do three things. First we will identify the best delivery method for the drug, for example, oral or injected or eye drops. Next, we will determine what dose of the drug is needed to elevate to level of protective enzymes in the retina. Finally, we will test the effective route of delivery and dose in the mouse model of AMD, employing some of the same techniques used in AMD in humans to measure protection, including measurements of retinal structure and function, and analysis of visual acuity. At the end of the experiments, histopathology will be performed to verify that protection has been achieved.
Our proposal is innovative in that we have access to large-scale screening used to identify drug compounds that offer retinal protection, and have identified several unique ones that have been tested for safety in cell culture and in animals. No one else working on the eye has these molecules.