Macrophage Origin and Function during Experimental Wet Macular Degeneration
Detailed Non-Technical Summary
Our studies are unique because we use advanced methods in a spontaneous wet macular degeneration model. We will perform a multiparameter flow cytometry panel for 10-20 color discrimination, which will allow us to identify macrophage subsets in a highly specific manner. In addition, we use state-of-the-art double hit mouse models to deplete macrophage subtypes specifically. These techniques will determine the mechanism by which different macrophage subsets stimulate new blood vessel growth during experimental wet macular degeneration. Our study is designed to detect macrophage subtypes that either increase or decrease blood vessel growth during wet macular degeneration. These results could translate into macrophage subset therapy. Cell-based therapies are advantageous over single-molecule therapies because they can target multiple pathways by which macrophages influence new blood vessel growth. By identifying unique markers for macrophage subsets, therapeutics can be designed to either deplete blood vessel activating macrophages or stimulate blood vessel inhibiting macrophage subtypes.