Inflammatory Cells In Choroid During Age-Related Macular Degeneration in Relationship to Retinal Pigmented Epithelium (RPE) Atrophy and Vascular Attenuation

Project Summary

The choriocapillaris (CC) is the only source of oxygen and nutrition for photoreceptors (cells that sense light) and RPE cells (cells that transport molecules between choroid and retina). The CC is severely attenuated in the region of RPE atrophy in geographic atrophy (GA), considered the late stage of dry AMD, and CC death in absence of RPE atrophy, which occurs in wet AMD. Mast cells (MC) are the effector cells of inflammation and immunity. Our preliminary studies suggest that MC degranulation is associated with RPE atrophy and formation of choroid neovascularization (CNV) in AMD. The MC degranulation spills enzymes and cytokines into choroid that could cause RPE and CC death and also cause the thinning of choroid, which is associated with both dry and wet AMD. The goal of this study is to document for the first time the number and activation of the inflammatory cells including MC, microglia (retinal macrophages) and tissue macrophages (TM) in choroid during AMD and determine their relationship to RPE atrophy and vascular attenuation. This is unknown and relevant since inflammation and complement activation have been implicated in AMD previously and the cell types assessed can contribute to a pro-inflammatory environment in choroid. If these cell types investigated in this study are increased in number or activation state in relationship to RPE loss, then this would provide new therapeutic targets in AMD.