Exploring the Role of Hepatic Lipase and Lipid Metabolism in AMD
Detailed Non-Technical Summary
To the best of our knowledge, HDL has not been investigated as a major source of druse lipid, nor has it been suggested in the literature that HDL may contribute to druse deposition in AMD in a substantial manner. Our study will establish if HDL accumulation results in RPE pathology and if modulating HDL results in efflux and accumulated cholesterol clearance. Well-characterized HL/EL dKO mice can be used as a novel model for studying AMD lipid biogenesis. Our study will reveal if modulation of LIPC can alter the mechanisms of cholesterol metabolism in RPE both in vitro and in vivo and if they can be associated with AMD by affecting cholesterol efflux pathways. Our study is not only innovative, but it will also extend the existing knowledge about the accumulation of lipid deposits as drusen deposits which are the primary hallmarks of AMD. They will provide insight into the role, mechanisms, and pathways regulating the cholesterol biogenesis in the aging retina and ways to intervene in this step for positive therapeutic application.