Domain Analysis of PN2/APP751Interactions with LRP

Project Summary

Dr. Knauer is studying the interaction of two forms of the amyloid precursor protein (also known as Protease nexin 2 or PN2) with the low-density lipoprotein receptor related protein (LRP). By binding to LRP, PN2 can be reintroduced to the interior of the cell, and there is evidence to suggest that this may be important in Alzheimer's disease. To understand how these proteins interact, the site of binding in the PN2 molecules will be mapped as well as the corresponding binding site in the LRP molecule. This information may be useful for developing molecular mimics, or agonists, that could prevent LRP-PN2 interaction, re-internalization, and, it is hoped, the formation of amyloid beta protein.